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Rapid Molecular Identification and Quantification of Allergenic Pollen By Real-Time PCR
Dennis K. Ledford,Mark C. Glaum,Michael Teng
- , 2016, DOI: 10.1016/j.jaci.2015.12.519
Abstract: Identifying and quantifying airborne pollen is important for causal assessment of allergic symptoms, for determining which pollens should be tested and for interpreting the results of allergy tests. Present pollen counting methods are time-consuming, require specific expertise and training, and are prone to subjective variability. Our goal is to devise a sensitive, high throughput method of quantifying pollen load in environmental samples.
3-Generational Familial Tryptasemia With Multiple Clinical Presentations
Dennis K. Ledford,Richard F. Lockey,Sultan Alandijani,Thomas B. Casale
- , 2017, DOI: 10.1016/j.jaci.2016.12.545
Abstract: To discuss a 3 generational multifaceted profile of familial tryptasemia.
Tacrolimus: A Heart Pill to Swallow
Dennis K. Ledford,Elimarys Perez-Colon,Lacey Harrington,Sweta Shah,Tara V. Saco
- , 2016, DOI: 10.1016/j.jaci.2015.12.082
Abstract: Tacrolimus-induced hypertrophic cardiomyopathy (HOCM) is a rare phenomenon. Tacrolimus-induced achalasia has not yet been documented. We present potentially the first case of tacrolimus-induced achalasia and concomitant tacrolimus-induced HOCM.
Combined fluticasone propionate and salmeterol reduces RSV infection more effectively than either of them alone in allergen-sensitized mice
Rajeswari Singam, Prasanna K Jena, Sumita Behera, Gary R Hellermann, Richard F Lockey, Dennis Ledford, Shyam S Mohapatra
Virology Journal , 2006, DOI: 10.1186/1743-422x-3-32
Abstract: Since the effectiveness of corticosteroids in treating RSV infection has been controversial, we tested fluticasone propionate (FP) and salmeterol (Sal) alone versus FP plus Sal (FPS) on RSV-induced airway inflammation. Mice were sensitized and challenged with ovalbumin (OVA) and infected with RSV. Following infection they were treated with FP, Sal, or FPS intranasally and airway hyperreactivity (AHR), inflammation and RSV titers were examined.The group treated with FPS showed significantly lower AHR compared to the group treated with FP or Sal alone. The group treated with FP alone showed slightly decreased (non-significant) AHR compared to controls. Treatment with FPS resulted in significant decreases in the percentage of eosinophils and neutrophils in bronchoalveolar lavage fluid and in lung pathology compared to FP or Sal. FP alone decreased eosinophils but not neutrophils or lymphocytes, while Sal alone decreased eosinophils and neutrophils but not lymphocytes. FPS treatment of mice infected with RSV in the absence of allergen sensitization resulted in a 50% decrease of RSV titer in the lung and a reduction in neutrophils compared to FP or Sal.Together, these results indicate that fluticasone in combination with salmeterol is a more effective treatment for decreasing airway hyperreactivity and inflammation than either of them alone in allergen-sensitized, RSV-infected mice.Asthma is a chronic lung disease with two distinct features – airway inflammation and airway hyperresponsiveness [1,2]. An association between viral upper-respiratory infections (URIs) and exacerbations of asthma has been reported [3,4]. The most commonly identified viruses in these studies include rhinovirus, coronavirus, influenza virus and respiratory syncytial virus (RSV) [5]. RSV is the predominant cause of URIs in infants below 2 years of age and infection may result in bronchiolitis, which is a risk factor for asthma [6-12]. RSV may constitute the earliest trigger for the development of
World Allergy Organization Guidelines for the Assessment and Management of Anaphylaxis
F Estelle R Simons, Ledit RF Ardusso, M Beatrice Bilò, Yehia M El-Gamal, Dennis K Ledford, Johannes Ring, Mario Sanchez-Borges, Gian Senna, Aziz Sheikh, Bernard Y Thong, the World Allergy Organization
World Allergy Organization Journal , 2011, DOI: 10.1097/wox.0b013e318211496c
Abstract:
Examining the association between maternal atopy and birth outcomes using a retrospective cohort in the southeastern region of the USA
Ayesha Johnson,Dennis Ledford,Hamisu M Salihu,Russell S Kirby,Thomas Mason
- , 2017, DOI: 10.1136/bmjopen-2017-017161
Abstract: Objective To assess birth outcomes in primiparous women with diagnosis of non-asthmatic atopy (NAA). Researchers hypothesised that women with NAA would have reduced the risk of adverse birth outcomes compared with women without NAA. NAA is defined as having allergic rhinitis and/or atopic dermatitis. Setting Women were mostly treated in primary care settings in South Carolina, USA. Participants This is a retrospective cohort study in which participants were identified using a Medicaid database. Participants were primiparous women aged 19 to 25. Births occurring between 2004 and 2014 were identified using the South Carolina’s Vital Statistics (VS) records of live births. Incomplete records (ie, information on plural birth, gestational age at birth or birth weight missing), plural births or infants born before completing 24 weeks of gestation were excluded. This provided 65？650 complete maternal–infant dyads, representing 97.6% of the maternal records and 96.9% of the VS records. Women previously diagnosed with NAA were frequency matched 1:4 to non–atopic controls for a total of 9965 maternal–infant dyads used in the statistical analysis. Primary outcome measures Low birth weight, small for gestational age and preterm birth. Results Linear tests for trend were statistically significant (p<0.001), indicating that NAA was associated with improved birth weight and gestational age at birth. After controlling for potential confounders, mothers with NAA had equal risk for each outcome when compared with mothers with no diagnosis of NAA. Conclusion A diagnosis of NAA among women living in the southeastern region of the USA does not reduce the risk of adverse birth outcomes nor does it elevate the risk of same. Additional studies with more rigorous designs are warranted to confirm the findings in this study
We Call for iCAALL: International Collaboration in Asthma, Allergy and Immunology
Jan L？tvall, Ruby Pawankar, Dana V Wallace, Cezmi A Akdis, Lanny J Rosenwasser, Richard W Weber, A Wesley Burks, Thomas B Casale, Richard F Lockey, Nikolaos Papadopoulus, Stanley M Fineman, Dennis K Ledford, the American Academy of Allergy, Asthma & Immunology (AAAAI), the American College of Allergy, Asthma & Immunology (ACAAI), the European Academy of Allergy and Clinical Immunology (EAACI), and the World Allergy Organization (WAO) G？teborg, Sweden, Tampa and Ft. Lauderdale, Florida, Tokyo, Japan, Davos, Switzerland, Kansas City, Missouri, Denver, Colorado, Chapel Hill, North Carolina, Omaha, Nebraska, Athens, Greece, and Atlanta, Georgia
World Allergy Organization Journal , 2012, DOI: 10.1097/wox.0b013e3182504245
Abstract:
Disease Cycle, Development and Management of Sclerotinia Stem Rot of Potato  [PDF]
Dennis A. Johnson, Zahi K. Atallah
American Journal of Plant Sciences (AJPS) , 2014, DOI: 10.4236/ajps.2014.525388
Abstract:
Potato is severely affected by Sclerotinia sclerotiorum in the US Pacific Northwest (PNW) and satisfactory disease management has not been achieved until recently. Recent research has given a better understanding of key epidemiological factors which has resulted in improved disease management in the PNW. This work reviews the epidemiology of Sclerotinia stem rot and highlights information that has led to the better management of the disease on potato. The primary source of inoculum of Sclerotinia sclerotiorum for potato in the Columbia Basin of the PNW are ascospores produced within potato fields or carried by wind currents from neighboring fields planted to winter cereals or other crops. Ascospores are ejected from apothecia and disseminated throughout the Columbia Basin over an extended time period. Immature apothecia generally first emerge at or shortly after row closure in potato fields in the Columbia Basin. Ascospores of S. sclerotiorum are incapable of direct infection of intact green potato tissues, and flower blossoms are crucial for infection and development of the disease in potato. Airborne ascospores are deposited on open potato blossoms still attached to the canopy. Infested flowers fall and are trapped on stems, usually leaf axils, or fall on the ground, and fungal mycelia then rapidly colonize the blossoms when humidity is high in the plant canopy. Ascospores are also deposited on senescent and dead plant material on the ground, germinate, and produce mycelium. Infection occurs shortly after contaminated blossoms become lodged on stems in the plant canopy, or after stems come in contact with contaminated fallen blossoms or decomposing plant tissues on the ground. Infection can occur within 3 days after contact of contaminated blossoms with green tissue. Lesions initially appear on potato stems 14 to 30 days after row closure and 12 to 20 days following full bloom of primary inflorescences of potato in the Columbia Basin. Significant levels of outcrossing sexual recombination have been found in the homothallic S. sclerotiorum population in the Pacific Northwest. Sclerotinia stem rot of potato is best managed by using an integrated approach of combining cultural practices that produce optimum plant foliar development without producing an excessive crop canopy, irrigation management to avoid excessive amounts of irrigation water, and timely applications of fungicides. Contaminated flower blossoms served as a bridge for infection and fungicides applied
Teaching InterMath: An Instructor’s Success
Sarah Ledford
Mathematics Educator , 2006,
Abstract: This paper discusses the InterMath courses from the perspective of an instructor. The instructor writing this paper was teaching her sixth InterMath course in the same school system at the time this was written. This paper describes a typical InterMath class and the success stories of many of the teachers participating in the courses. The instructor also reflects upon her growth as a teacher during her experience with InterMath.
Approximating Continuous Functions with Scattered Translates of the Poisson Kernel
Jeff Ledford
Mathematics , 2013,
Abstract: The goal of this note is to show that continuous functions may be approximated using scattered translates of the Poisson kernel.
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